Loading...
HomeMy WebLinkAbout1993 Water Quality in the Ludlow Watershed 1991/1992WATER QUALITY IN THE LUDLOW WATERSHED 1991 -1992 Prepared by Glenn Gately Jefferson County Planning and Building Department Port Townsend, Washington 98368 for Washington State Department of Ecology April 1993 WASHINGTON STATE 0EPAATMENT OF ECOLOGY This project was funded in part by Washington State Department of Ecology TABLE OF CONTENTS ACKNOWLEDGEMENTS ............... .............................ii INTRODUCTION .......... ............................... .. ..1 Oak Bay Basin ..................:...1 Ludlow Bay Basin ...................1 Paradise Bay Basin ............ ....4 Bywater Bay Basin .. ...............4 Squamish Harbor Basin ..............4 METHODS........................ ..............................5 DATA INTERPRETATION ............ .............................12 RESULTS........................ .............................13 Oak Bay Basin .....................13 Ludlow Bay Basin.. ..............18 Paradise Bay Basin ................18 Bywater Bay Basin .................20 Squamish Harbor Basin .............20 DISCUSSION ..................... .............................22 Oak Bay Basin .....................22 Ludlow Bay Basin ..................24 Paradise Bay Basin ................26 Bywater Bay Basin.. .26 Squamish Harbor Basin .............27 REFERENCES ............... ............................... ...29 APPENDIX A: Quality Control ..................... ..........30 APPENDIX B: Sample Site Locations ................ ..........35 i ACKNOWLEDGMENTS Appreciation and thanks go to all those who contributed to this study: Debra Bouchard for planning the study; Pat Rubida for surveying streams and collecting samples; Brian McLaughlin for surveying streams and collecting samples; Teresa Barron for helpful suggestions; Craig Ward, Shirley Van Hoover, and Lynn Krum for administrative support; Mary Mandell and Laurie Frey for speedy and accurate typing; Tim Determan (Washington Department of Ecology) for technical support and reviewing the draft; Gerald Lukes (Washington Department of Health) for providing marine water quality data and reviewing the draft; Stewart Lombard (Washington Department of Ecology Quality Assurance Section) for reviewing the draft; Tom Smayda (Vasey Engineering Company) for reviewing the draft; Cecelia Larsen (Ludlow Watershed Management Committee) for her excelent job of editing the draft; John Merchant (Port Townsend Sewage Treatment Plant) for allowing us to use his laboratory; Craig Hanson (Kitsap Sewage Treatment Plant) for excellent service in analyzing fecal coliform samples; and The Ludlow Watershed Management Committee for their helpfulness and patience. ii INTRODUCTION This report summarizes the freshwater monitoring conducted in the Ludlow Watershed by Jefferson County Water Quality staff during 1991 and 1992. The work was partially funded by Washington State Department of Ecology Grant TAX90218, a Centennial Clean Water Fund Grant. The Ludlow Watershed, which comprises about 23,000 acres, is located on the Olympic Peninsula in northeastern Jefferson County (Figure 1). It is divided into five basins (Figure 2). Oak Bay Basin Oak Bay Basin at the north end of the Watershed contains 4,664 acres of gently sloping forests. It is transected by several, small, unnamed tributaries which flow into Oak Bay or Mats Mats Bay. The western shore of Oak Bay is lined with houses. There is a County park at the northern end of Oak Bay. Agriculture in this basin is primarily limited to horse and cattle grazing in pastures around Mats Mats Bay. A rock quarry, located on the east side of Mats Mats Bay, produces about 300,000 tons of crushed rock.per year. A herring bait facility is also located on the east side, where herring are held in floating net pens for about two weeks prior to packing. Mats Mats Bay has a public boat launch and moorage. In October 1992, there were 367 acres approved for commercial shellfish growing in Oak Bay. Recreational shellfish harvesting occurs on county land along the south shore of Indian Island and in Oak Bay County Park at the north end of Oak Bay.. Geoduck clams occur in the bay. In Mats Mats Bay, oysters are grown commercially in the north end of the bay. In 1992, 15 acres were approved for commercial growing. Ludlow Bay Basin Ludlow Bay Basin, the largest basin in the Watershed, encompasses 11,085 acres of mostly forested lands. Ludlow Bay Basin has the highest population density of the Watershed. A resort and marina are located on the north side of the bay. Facilities include a fuel dock, hotel, restaurant, grocery store, marine supply store, and public restrooms. A yacht club also has a dock on the south side. Although not located directly on the bay, a golf course drains into it. A log dump -and- storage operation at the north end of the Inner Bay has typically contained 5 -7 million board feet of predominantly Douglas fir and red alder with lesser amounts of western hemlock and western redcedar (Jeff Grant, Pope and Talbot, personal communication). A wastewater treatment plant discharges about 0.1 million gallons of treated effluent into the Outer Bay each day. 1 • RSHED N • i0 20 ss- hatched area) in 2 .t }- '� •�:, :a • �;� .,` Port Townsend. •� Fort Angeles LUDLOW WATE JEFFERS O Seattle COUNTY • Tacoma Vi cin ity Map SCALE: �( Miles 30. �vUK Figure I. Map showing Ludlow Watershed (cro eastern Jefferson County. • RSHED N • i0 20 ss- hatched area) in 2 Woad Womd Marrowalons 61add / t / `Oak Ludlow Watershed � gay Basin \r �+ z/ //. ` Ludlow Bay Basin Pgra s Bay , Squamish Harbor Bas � � ! � Byw4 1 / Basin j Bay Legend Watershed 8 o u n d a r y —' — Basin Boundary S a o I i : 1 lack ■ 7950 toot 1115/91 Figure 2. Map showing the Ludlow Watershed and its five basins, 3 Ludlow Creek is the primary source of freshwater to Ludlow Bay. The mainstem is 4.5 miles long and has 33.5 miles of additional tributaries, including intermittent ones. Ludlow Creek drains about 8,600 acres of mostly forested land. Animal grazing occurs at the headwater of the North Fork. Several smaller, intermittent drainages also flow into Ludlow Bay. Paradise Bay Basin The Paradise Bay Basin is composed primarily of 1,306 acres of steeply sloping forested lands. Most of the residential development (Paradise Bay Community) occurs near the center of the embayment. The remainder of the basin contains scattered houses and several unnamed drainages, which all flow east into the Bay. Geoduck clams occur in the bay. Bywater Bay Basin Bywater Bay Basin has 957 acres of gently sloping forested lands with scattered residential dwellings. The southern portion of the Basin is crossed by State Highway 104 with access to the Hood Canal Bridge. To the north of the bridge is Bywater Bay State Park, which has a saltwater beach and public boat launch. At least one unnamed, intermittent tributary flows into the Bay. Shellfish are grown commercially in the middle of the bay. Recreational harvest occurs on the Wolfe Property (State Park land) at the north end of the bay. Squamish Harbor Basin Squamish Harbor basin drains 5,182 acres of gently to steeply sloping forest lands. Residential development is confined primarily to the shores of the harbor in the communities of Shine and Bridgehaven. The Shine Rock Quarry, located three miles west of the Hood Canal Bridge, produces 72,000 tons of basalt rock annually. Shine Creek, having 17.2 miles of mainstem and tributaries is the major drainage system of the basin. This stream originates on Pope Resources' golf course and flows through mostly forested land, although much of the land has been recently clearcut. Several small, intermittent streams also empty into Squamish Harbor. In 1992, there were 1,450 acres approved for commercial shellfish growing and two commercial growers. Geoduck clams are intermittently harvested. Recreational harvesting occurs at three locations in Squamish Harbor. METHODS Ambient and storm event water quality monitoring was conducted on streams within the five basins of the Ludlow Watershed from February 1991 to April 1992 (Figure 3).. The parameters measured were: flow, fecal coliform, total suspended solids, conductivity, pH, temperature, and dissolved oxygen. Flows were taken at the downstream sites. Ordinarilly, stream velocity was measured with the use of a model 201D Marsh- McBirney current meter and stream flow was calculated by the summation of incremental partial discharges across the stream. When the stream was too deep to safely wade or too shallow to cover the meter probe, flows were visually estimated. Such estimates are designated by an "e" in Tables 5 and 7. At site LD1, where Shine Creek passed through three 3 -foot diameter culverts, stream flow was obtained by summing the discharges from each of the culverts. Culvert discharges were calculated by multiplying the cross - sectional area of the water occupying each culvert by its velocity. Velocities in the culverts, as well as in the typical stream reaches, were taken at six- tenths the total depth as measured from the surface. At two sites (LD6 and LDS) flows were usually calculated by a method in which the flow was caught in a container of_known volume while being timed with a stopwatch . Both methods used are described in Guidance for Conducting Water Quality Assessments (Anon. 1989). Fecal coliform samples were collected about 6 inches below the water surface in 100 ml sterilized polypropylene bottles, transported on ice, and delivered to a State accredited laboratory where they were analyzed by the membrane filtration method within 30 hours from the time of collection. Lost samples (due to poor commercial bus transportation) necessitated changing to a different State-accredited laboratory during the study. Samples were not analyzed if the 30 -hour maximum holding time was exceeded. Total suspended solids (TSS) and turbidity samples were collected in 500 ml dark, polyethylene bottles, transported on ice, and refrigerated. TSS samples were analyzed within 7 days and turbidity samples within 48 hours of collection. The analyses were conducted at the Port Townsend Sewage Treatment Plant laboratory (State accredited) by County staff. TSS was analyzed by Method 209D and turbidity by Method 214A (APHA 1980). Turbidity samples were analyzed on an Engineered Systems and Designs' Model 800 meter. Temperature, conductivity, dissolved oxygen, and pH were measured on -site with a Cole- Parmer Model 5566 Water Analyzer (manufactured by ICM Company, Portland, Oregon). Conductivity measurements were meter- compensated to 25 0C. Dissolved oxygen and pH results are not reported because variations in replicate 5 ./) I - Is. LD11 -1 OAK LEGEND 13 a ( - 0 Ambient ■ Storm event A Ambient and storm event 1=1 , 'I- Z" I I I I I I I I,=- \j t."A-M MAT'S 1 AXSA L08 LD21 06-1 LD7 ' \ .... LN L U Lv I A LUDLOW L f %: SAY LD2 L031 f�� I ( LLD FARA1715;F— FAY f f __ _ - LD41 N' LD51.. KEY I -1AP D1 ub" A4 Part ro.nieltd HA 809 P\ E" PitaJECT AREA Hood CXnall"', Figure 3. Map showing ambient and storm event sample. sites, 6 samples made their accuracy suspect. At the conclusion of the fieldwork, the Water Analyzer was returned to the manufacturer and the probe was found to be faulty. Laboratory and field replicates and QC sample results are reported in Appendix A. Rainfall data was measured at Center, approximately midway between the north and south ends of the Watershed. Data are reported in Table 1 to help interpret the results. However, large variations in rainfall within the Watershed were sometimes observed. On some dates heavy rainfall was observed at one end of the Watershed and no rainfall was observed at the other end. Thus, this data may not be representative of rainfall at the sample sites. Water quality criteria for the various classes of waters and their beneficial uses are given in Water Quality Standards for Surface Waters of the State of Washington (Chapter 173 -201 WAC). Criteria for the parameters are summarized in Tables 2A and 2B. Although all waters monitored are designated Class AA (extraordinary), criteria for the other classes are given in order to allow one to assess the extent of impairment when Class AA criteria are exceeded. Beneficial uses for the various classes are given in Table 3. The State fecal coliform standard has two parts: (1) the geometric mean value (GMV) of the samples should not exceed a certain value (dependent upon the class designation); and (2) not more than 10% of the samples should exceed another value (Tables 2A and 2B). If either of these parts is not met, the standard is violated. 7 Table 1. Rainfall accumulation at Center, Washington in relationship to ambient and storm event sampling dates. Sample Sample Rainfall accumulations date type 1 -day 3 -day (inches) (inches) 1991 February 13 Ambient 0.09 0.59 March 13 Ambient 0.00 0.27 August 22 Ambient 0.00 0.18 September 17 Ambient 0.00 0.00 November 25 Ambient 0.06 0.21 December 17 Ambient 0.10 0.10 1992 January 27 Ambient 0.87 1.73 February 19 Ambient 0.21 0.28 February 24 Storm event 0.00 0.12 March 9 Ambient 0.00 0.00 April 22 Storm event 0.00 0.90 1 Accumulation period ended at 0900 on the day of stream sampling. E:l .4 e-i O N I M t` H U Cl 4J ro m c O 41 0+ A N ro 3 a 0! 34 a+ CO to c .0 'C N S4 W O w� 41 w x O 0 N v ro� N N ro N -1 N U ro EnU 010 �4 4-1 (a ro > 01 A N 4J v O Q) 44 ro ••% TS )4 N +� N � O U a .3 41 o w ,••1 010 �a )= A 0 +1 N 0 -M N N QJ � 3 rod E b w TA a W E y W a P4 i N O c .0 o w 11 tll •.i w 'L1 � O a U -4 U .. coo c 4J O C c ro aci 4J � v 3 �4 O E A N N H >4 ro J a �4 a~ 7 ll +t 4 ro tU O 04 a N 3 +1 ON � c > a X N U �4 ro A � tl) A 4 N A 3 � , . ,-4 ro � N � � 0 v N O N ro "•i U N 4j y U E 0 �4 4J 0 O 44 0 'r. t0 N ro 0 4J c N E •..1 . Q) 'd 4 -4 ul ro N >a ro L4 7 -4 $4 w 4) iQ O M a� aa) H � U U F O 3 .a 1 c ro E m � a N N tU ro ro N ,--I rl � .•. r4 ro c0 4J U� U U ro+ U tr • r- N•.1 N N •.E E O O N ro ro ro U.. .ro row O O OT O to ,-4 v d v N O 1 N U ); N N v to 7 0 ro ro n ro _ to to VI 41 A %O U V v c H0F w z s4 z tr >4 H Ln U Ln > to O 0 VIAA O N rl N 'O it 't3 N wk 000304 ro NO Me N O 0 E o H -ON U rots O ro0 0.50+07 U+ ti• •-+ �- N N rl 9 E �4 -H O o E+ E N U U Z U W O— 0 VI uW U ro (0101- OT Ul Ow AOA o-G H sr N 1-4 O e 4 N U N M ID 0 `,f,• O W w r0 I) (0 U VI d' H VI •r1 VI A VI 4= A to w t 0 ro 0 A N m U�.-t U c++ N r•I w 0, E U+ t r it ro -4 N .-1 O E O N O E E .0 0 ro ro O N O ro O • +U 7 ,4 U .•1 N r-1 LO U k -- 03 co co • •O to N VI dB U E .-I N co co N 044 ro II I N O r4 O N VI 41 A to 7 o . ro a VI N �o U v ` 'C7 E-4 0 z 0 tno v 0 Ln x to > ro >4 -4 U O E VIAroA ro oA O. •.li r1 N c 3•+ 0 0 '0 --,4 -4 7 N 7 N ro c r. 4-J N O w E 0 1.r O w U c to 0 roro oroo 0+ O+c7 u+ to N r+ 9 D .�4 _4 O o f E to C-) 4J 1- UE U P o-- •'0N X VI 40 U ro Z ro x tr . %� to co v 0O AtArl 0 n U r1N O% In 7 - o w w ro u ro 0 Vt ri H A •.4 Vt A VI 41 A %O U V 0 N _ i1 >, 0 W Q .14 d N O 41 4J U N b S+ 1-1 y cE0 b .0 04 >a U �4 E N A. w w E E+ O i N O c .0 o w 11 tll •.i w 'L1 � O a U -4 U .. coo c 4J O C c ro aci 4J � v 3 �4 O E A N N H >4 ro J a �4 a~ 7 ll +t 4 ro tU O 04 a N 3 +1 ON � c > a X N U �4 ro A � tl) A 4 N A 3 � , . ,-4 ro � N � � 0 v N O N ro "•i U N 4j y U E 0 �4 4J 0 O 44 0 'r. t0 N ro 0 4J c N E •..1 . Q) 'd 4 -4 ul ro N >a ro L4 7 -4 $4 w 4) iQ O M a� aa) H � U U F O 3 .a I n U 3 ar JA ro N C O W IT C N C N ?� >4 a) ro 4J c 3 � l4 a) O 44 4J U ro O N N ar w N it to r W U O N ate) (1) JJ N ro ro ZT rl •.•I U N to O � rt > 'O .0 N 41 3a +OJ w3 I O {J 0 ••4 .] w U a) v +) 4 9 W o U )4 JJ 3 U2 U ro W 14 N (A a) .••1 A ,t ro E 0 N u� N •'i (a W U N O -roi U+ U W ' E H K a) ri N r-1 N a) -ro+ k U a) Lr N O U k a) )a w yJ W ro c� ro L4 ro ro a�4 i U b C 4 N NN O )a •i 41 � fu � .� w f0� ,-� to > Nro E C 0 U U U C 01 tn N a) NO N ja N N a In 0 O }I r1 ro ro ro U1 U) VI JJ n 10 U v }O) O JJ Ems+ 0 H C 'J) z 01 Z Sa 11 In U Ln > � (a O c i o vt .0 n o a) N a) a) r/ N rl a N R5 N ri W r-4 r 0NCro C N ro O .Q 44 o ooUO (a O�J ro MOO r+ ,4 O g w >4U -- U y W 0 o ro-� M D 17%9 0 U O+ a) rI ri N U X E X ••4 O O !~ N N fs. rl w U z U )•i O E VI ow o (d (a aP is O O a) 0 ,o0Ja0SG ON tD a) 4J +3 a) rl N 14 N b In rd J» w OJ +J - U VI n H VI ••-i VI 11 VI ,1J A N N b � O 3 4J tT a) ; Table 3. Beneficial water uses (denoted by "X") of the different classes of surface waters listed in the Water Ouality Standards for Surface Waters of the State of Washington (Chapter 173 -201 WAC) . FISH AND SHELLFISH Salmonid migration x CLASS BENEFICIAL USES AA A B C WATER SUPPLY x Salmonid spawning Domestic x x Industrial x x x x Agricultural x x x STOCK WATERING x x x FISH AND SHELLFISH Salmonid migration x x x x Salmonid rearing x x x Salmonid spawning x x x Salmonid harvesting x x x Other fish migration x x x x Other fish rearing x x x Other fish spawning x x x Other fish harvesting x x x Clam /oyster /mussel rearing x x x Clam /oyster /mussel spawning x x x Clam /oyster /mussel harvesting x x Crustacean and other shellfish rearing) x x x Crustacean and other shellfish spawning) x x x Crustacean and-other shellfish harvesting) x x x WILDLIFE HABITAT x x x RECREATION Primary contact recreation x x Secondary contact recreation x x Sport fishing x x x x Boating x x x x Aesthetic enjoyment x x x x COMMERCE & NAVIGATION x x x x 1 Crab, shrimp, crayfish, scallops, etc. 11 DATA INTERPRETATION Fecal coliform results are interpreted by comparing geometric mean values (GMVs) to State standards (Tables 2A and 2B). The Washington Administrative Code (WAC 173 -201A) does not specify a minimum number of samples from which to calculate a mean value. The Washington Department of Health (DOH) has a policy of calculating a GMV from a minimum of 15 samples. There is good justification for having a large sample size because fecal coliform concentrations are highly variable. Sample sizes in this study are small, especially when dry season and wet season samples are considered separately. Despite the small sample sizes in this study, comparisons have been made to the standard to help the reader evaluate the data. Caution should be exercised and sample size considered before drawing conclusions. For the same reason (small sample size), fecal coliform loadings must also be interpreted with caution. No State standard exists for evaluating fecal coliform concentrations in shellfish tissue. Studies often reference a 230 fc /gm Federal (Food and Drug Administration) guideline. This guideline was intended to assess shellfish marketability, for which purpose shellfish are tested after processing. The 230fc /100gm level is used by DOH (1990) in the Puget Sound Ambient Monitoring Program to place shellfish in the most contaminated of three categories (<30, 30 -230, and >230 fc /100gm). Thus, reference is made to this 230 fc /100gm concentration in evaluating data cited in this study. As in water, fecal coliform concentrations in shellfish tissue are often highly variable and sample size.must be considered in the evaluation. 12 RESULTS Oak Bay Basin Three unnamed tributaries of Mats Mats Bay were monitored under the ambient sampling program and a fourth tributary was sampled during storm events (Figure 3). Samples from all three ambient sites (LD6, LD7, LD8) exceeded the 50fc /100m1 State standard on some dates (Table 4). Fecal coliform levels at all three sites were highest (range 240- 540fc /100ml) in samples taken January 27, 1992. This date is associated with the greatest rainfall of all the dates sampled (Table 1). A fourth Mats Mats Bay tributary (LD21), sampled during storm events, -had almost no fecal coliform bacteria on the two dates sampled (Table 5). Site LD6, sampled on the same dates, averaged 67fc /100ml. During the dry season (sample size 2), sites LD6 and LD7 failed both parts of the standard (Tables 2A and 6). During the wet season (sample size 6), sites LD6, LD7, and LD8 had GMVs below the 50fc /100ml limit, but failed to meet the second part of the standard ( <_ 10% of the samples > 100fc /100ml) . Flows for all four Mats Mats tributaries (sites LD6, LD7, LD8, and LD21) were always low, never exceeding 0.5cfs on any of the dates sampled (Tables 5 and 7). Thus, fecal coliform loadings for these tributaries were comparatively low and usually did not exceed 0.1 billion fc /day (Tables 5 and 7). Total suspended solids (TSS) and turbidity were highest at ambient sites LD6, LD7, and LD8 on January 27, 1992 when rainfall was greatest (Table 1). On this date TSS ranged from 47 to 127mg /1 and turbidity from 63 to 88 NTUs for the three sites (Table 4). Storm event site LD21 had a TSS value of 38mg /1 on February 24, 1992 (Table 5). Temperatures in all four Mats Mats Bay tributaries remained below the 16.0° C Class AA limit. Conductivity ranged from 77µmho /cm at site LD21 to 225gmho /cm at site LD8. Besides the four streams flowing into Mats Mats Bay, one additional stream in the Oak Bay Basin was sampled. An unnamed tributary which flows into Oak Bay, was sampled at site LD11 on two storm event dates. The mean fecal coliform level for these dates was 17fc /100ml. Both parts of the fecal coliform standard were met. Of the eight storm event sites monitored in the Ludlow Watershed, site LD11 had the highest flows (mean flow 2.0cfs; Table 5). 13 N co 1 %0 1 I I in o ao M a1 ry N to M In Aa to N a 3 •.� '� r 1O r- N M O > R r. c0 1 I 1 'i M .--I 01 r N 4\ o r I I I N to -W r ko N r4 m O N a I r-1 '•1 ri r ri M r•1 v V' r•1 o Z ,�" •i4 •� . '� r o 1 1 t M 111 r-1 ro 9 41 N M F a N to 0 ... (a t] co N Ln co %o ri co c• d- co c0 01 , a� E N %D H I M to O 0 M to m- ro N ' 4J 111 Aa to N a `� '� r 1O r- N M ro to I c0 1 I 1 'i M .--I 01 r aI W I 1 c0 w r In co m 4 o ,�" •i4 •� . '� r o 1 1 t M 111 � M co N M F a N 1.4 ro r-1 co N O Az c• , a� E N %D H I M to O 0 M m- ro b E w M %D In r ry '•1 (a a o r 4 in 14 14 m to 4J 111 to N a `� '� r 1O r- N M 1 I I 4J >1 m 4 ,�" •i4 •� . '� r o 1 1 t M 111 � M co N M F a N 1.4 N r-1 co N O Az E N %D H I M to O 0 M O ro H 4 m >11 co N H I I 'd' O N N l0 ro a M 1 I .-1 N H N 1•1 A >s 4 -4 '� D 1-1 H '-I W r N d ro G m 'c a o 1 s� r ,•; rn r 1 io t. w 4J at o 1:4 -4 s+ E w r-1 c z 4 N fA w M O O k0 01 O r C V R •' c-; � M v M , N ,d v co o t I 1 W n %0 co m a cr I 1 1 m N N to �4 O r O O %0 v row° `O ; c° ' 1 ,°a H cm G*. O w V v $4 m 13, %0 a 0o t o 0 0 %0 o co r- N +-3 •.i '-1 ro .. O � 01 01 .-1 01 N 01 a7 M 01 H 14 H 01 14 01 01 01 '-1 01 01 01 -1 4J 4J 0% 01 01 r4 r H 3 7Oi ro M r•1 ' 1 rn a N f1 N N d G4 A ri 03 >� >a 01 v N N 0 m C f1. Sa U 3 +1 a) 7 sa U w z a E to aroi h w z .1 i N >a v A v O z m LL i; ro N N fJ a) 4 a) ro sa m a s4 Q) w O N �4 W A 4 a1 O z C O G a1 x • ro m N -4 m 4 1,7 > w U O C O U b ro QJ O 41 ro as d LL R a) E .r 9 C .••1 m N 1 n N O to 0 > M tD n O1 I in tD to r- 0 O .. \ o 41 1-1 � $4 %0 N1 n I N ON co n n yl O tn Q 00 v O 1 %D n to co In ❑ U N a' ri '•I rl .-1 r•1 14 ri a C .Q) a ZL 41 id N1 a b A In c1 n c0 r1 v N N O d m N n v to v v tD 41 -4 w H '•1 H '•1 H H '•1 C a C O c0 14 c0 IT IT N 14 41 O1 O O I O O) O O1 A v 1D r1 n to to 0) I -. 0. 0 co I (n 00 00 0) C x N 01 Ci Ny A v N In n v O N co � Ey w •.�•1 co n n ao O co co 0 to m 01 ul C 111 H N 1 14 N .-1 e-1 ro r1 .i N 1 H N %D '•1 '>7 0 N � N >1 O O i-i .•. .14 .-. :1 O In 111 1 ri .-i r•1 14 '•1 I ri N n O1 1+ I.1 11 E 14 °a �4 v T! EO.e A O v co (1 O N P1 C>1 tD N N H 14 N 1f1 ri ro0 w w Rl d' N it al C v M n c•1 to ?c 4J ro n i ) 01 tD N 1 tp O v v ro 3 � � N A LLN.. co v to v C .,4 a o tD I N 0 rl 2T v ri H I n .-1 v v O ❑ 1 N a tT 4 A v 00 00 0 o N o tD C CS O N c1 .H % E.c w m N ko at v v co tl. ro c 1 F." tD M H t0 01 01 111 N❑ ro 11 c0 O 1 11 v O O b I 4j � O O O O O O O N >. W •+ — > ro .,.1 Cat N 111 N '•1 cn O1 N n 0) 21 1 C C 14 O 01 O 1 O n O O 0.,, 0 a U •.•1 O e-i O O O O O •.i N ro '-1 31 t0 ro -1 .,1 O tD r1 '•1 N 14 '•1 111 ro U A A In M O- O O\ t•1 M O c m O Tl C n n H 1 n N t11 00 '•i ro tD � I N C• Q) °a U (: r-1 0l O c•1 j+ E $4 r1 rl e-1 I D d r 1 01 r1 i-1 0 0 O. t`1 v v I tD �-+m N cOWO Kc ka U C% ❑ W O U ro a ••'1 U w A to n H m v 01 111 co 4j — N m rl N N N to w .-+ H 10 >4 C ro -H >4 co C ri tD N IA co tD n 41 C ro rl O c1 O N V n N •4y3 al 'i 41 0 ,%„ O N O O O O O O to to A CT 3 N ko of m v m N Cl O W w O 01 m '•I 111 t•1 1.1 iJ W r•i U a o o� al ro ro w — 04 o a o 0 0 .+ o ro N 3 N to ri v O to r+41 A H rf v O m V• 111 N e-1 •.1 01 • •-1 !n w. O N O O O O O O to ❑ --4 1 A �+ F. )✓. +1 tD 4 N N v I11 td n c0 ro 10 ro"1 ❑ ❑ ❑ ❑ ❑ ❑ ❑ ❑ E4Mvl In 1n a a a a a a a a 15 A •Ci N i� ro C N N b Ol 3.+ ro N tr G 4 O I ^'1 t. C' +C O a. N• a� it sa 0 U C ro N 3 O 14 w Ol 41 ro 4J N W .4 3 O •c N N t7 1•i `. ro W N 7 QI > N � tU ro r, N U y -4 1•i 41 Q) a4.Ji O v tT N .ri WD rT � c li ro ro c . ro z N �ro 4) 41 N ro .i J-1 N U1 tH N ri 4 m +1 Iii rd LI N O c 0 N C li O d N � ro4-3 vv N 3 Al r_ c IO .•i O 4 O d r�l 3 U w li O 1-t c O cd (4-4 C W N S.i •ri IOJ ro to b+ N � ro ri N s b 0 N O 0 W M r f tT 4) U 4J � C tT ri tU •ri ro U k. N N C w a•ri �O N ri A ro H N n• N 41 0) W O tr ri o O A r•I N 0 U c w 0 N to N r4 N B O r+ o U W 61 � 2Ji E c O ro O a �+ \ U W z N V a) J•1 N UI z r-t rti O � f•+ ri O < A U OW tN 0 r-I z s 0 0 0 U-- N U ro w v N � � 3 r- •Oi a U w z N }.1 n• N b W J.1 £ VI 41 r4 N O I; tn00 1 ri O A \ ri O o N O NU'\ N U W $4 1-1 • G O ro ri a\ U w z r•1 ro >,•ri N N N 0 0 0 0 1 1 1 1 1 1 1 1 wwwzzzz 0 0 0 w w to n 1 1 1 1 1 1 1 1 M MN ri wMinmwmw ri ri ri N C r•tN O �t11O000M 01 0� tt1 0� d' v n I 1 1 1 I I I I I 1 1 ri to NN v ri M I I I I NnMe1• V w w w w N N N 0 0 0 0 O N N O N N N v N w z z z z z N w I z W w o >•t >+ > >i >+ » >I >t 000x, n 00000000 ri H H ri to to nwrir-IM0ID nn. - I I nNtnw ri r•I ri M ri N t0 H N V' ul kD tn QOM V -w r- riH ri N10 tom a%N MMtn N N 1 v 1 Mrs I N 1 { 1 I I I I 1 M 1 r•1 1-1 1 W 1 w V• r♦ M N n M dr M n V 'O N ri V %D %D %D to %a W%0 NNNr•4 NNNN N O N 0 0 0 0 z N z z z I 1 1 1 1 1 1 1 1 >+ >t O O O O O O O O to I I I I l l i t l ra ra ' H to M ri to r4 O ri N 1 1 I 1 I 1 1 L 1 ri %D C W r-i r•i O coo to a%N 0\ v 0% to ri ri ri N 1 1 1 1 1 1 �Od•OOO 1 1 1 1 1 1 1 I. I tn�•Mrnr•tw ra ri N N N N N N I 1 1 1 1 1 I t I ,.1 C N 11 > C N L r♦ ri ri ri r•I ra r♦ •rig -i NMC%O nw c r•I N M"W m 0r, 4Oin in aaima •riaa0Q0OIm O aaaaaaa aaaaaaaaa 16 1.1 O c 3 ri O O \ N E N C ro tr is O O tn c ro i.i N .L.1 ro ro :4 1r J-1 O W O ro > ro tU U t•i +1 tv O tT � 0 roO ri Fi N y •ri y1 C ro ro -- �4 N 0 U � p W O r•1 C ro N .0 N d1 . 14 {-I . U � ro 44 Q N .roty t0 � � ro N N !V O 4j W r1 N-1 ri O O U ^w 0 r•I U C Ol ro W C W N N N k 17 . L p to rn � O O N ra I M c't I O O y co I 1 I t O O to O r'1 0 � a .4 a I I t 1 o o N o 0 o O n Ul 41 Ct o� lh N U) t•1 d' ON IS n 1 'r to .4 1 r-I O O O O O O 0 O O N O O O O O Q) w 10 w O ip M N t O 01 r- N O V' e•1 r4 r-1 H 41.4 r4 O 01 O �O r♦ O .-I ri C 3 0 0 1 1 O O O v O O O O O tO 0 W 0 O C .,c -+ N rl co N d 0 U -•I N 1 U1 co ID co C N N N Hi fO ri A a �O 1 N N p r-I ri N O .. U) U N) W oa t %D r y v n .4 0 Lo to Q) a �1 N rl 1 N f r'1 N Ln m O O to N U O in 14 1 N '1 C . 3� O 0. r-1 r ril v o r d w ra .a co c o m ra o ri I p ,a I 1 I I 0 1 1 o O o 0 0 1 0 0 �. N ' r♦ a% 4 y N N � ko !fl t, w m 1Qa m C'1 tb O O O O •"1 o O H a 1 1 0 0 0 0 0 0 0 0 0 0 N t6 N 4) 41 .� e 1 C d O \O to N n o CA r�-1 o C 3 rn 1 .� o o v o It .�� 0 1 0 0 0 0 0 0 us a% PW ,4 o >, ro W W (C N !` N N N N 1-1 to T-4 r- t� N a% _ L y N N 3 S4 r7 N{ N 14 � `r � `� � f4 0 OA v•rl 1-1 Q) - 1Q SQ 11 b I~ r 1 0 1` co O '-1 N v OI n C ri r I 41 >4J d ('1 ko N N r- 10 is ra cn M b Z v w G U tP >~ G N O 1 O O O I 1 1 � V' cn %a co �O 1 %D 1 N N N U t17 TJ •.i i]. ►a v 1 I 1 r� l� O N 4 W E W b N '-4 CO %0 1 O O to d' O O M c'1 O In 0 IT U a a I Q\ co o. Q) N •rd . U TS W tO -V r, C N ••1 U N O 0 O r-1 N >a a U r-I O W co o� off H ° v i Ln m "' d U Q))' �o w o a cov c ra 4J U 0 0 0 •i H Ln .r4 to (D a ►] W 14 b U r-i 0 .r4 G N O U N • 1 O U W N O I %a to ' N � Qw C) s to — (a )1 -.-1 to C) 14 U w (a �N O -rd 4J U 0 i4 4J U 4) U .� �O I d' In N co %O �D v r-I n `4 H 'II '� ja W A e4 I N V ri rn C v td r-4 41 to tO > O z to 4)i to W CO N rN 3 r. ON rO.1 o v y r 4 14 CA 14 H 0 r•i r-1 N N a% N G 1� G 44 I� J, a\ w a% O\ a% 01 -1 rn rn rn o+ O m O>+ 27 N U y 0 b (A 14 rn m ri ri to ri r♦ ra 14 m ro (0 P 1-1 N m 4J tO 1 d I 4J (d JJ 4J c') i l: Q1 N1 N r'1 r-4 cq N Ey H ri N N ri N 14 a% N >r o 41 0 4J 0 O G Ha Nx m 14 t' A 1J >T f1 > U !; A Sr 10 A r-1 W W C7 y W U1 Z o h G4 = i 4 M r M 17 Ludlow Bay Basin Results of the ambient monitoring conducted at three sites on Ludlow Creek (Figure 3) are shown in Table 8. Fecal coliform GMVs were higher during the dry season than the wet season. Dry season GMVs for sites LD2, LD3, and LD4 were 66, 41, and 601 fc /100ml respectively, compared to 8, 11, and life /100ml for the wet season (Table 6). The worst conditions during the dry season were observed at upstream site LD4 (GMV 601 /fc /1OOml). Site LD4 failed both parts of the fecal coliform standard in the dry season and the second part in the wet season. Additionally, site LD2 failed to meet the first part of the standard during the dry season. Mean fecal coliform loadings were 2.6 billion fc /day in the dry season and 2.0 billion fc /day in the wet season (Table 7) . TSS was less than 8mg /l on all sampling dates except on January 27, 1992, a date associated with high rainfall (Table 1). On this date TSS measured 53, 12, and 39mg /l for sites LD2, LD3, and LD4 respectively (Table 8). Similarly, turbidity values on this date (55, 14, and 60 NTUs) were also higher than those obtained on other dates (<5 NTUs). Temperatures at the three sites ranged from 1.8 to 14.7 0C_. Conductivity at sites LD3 and LD4 ranged from 99 to 164µmho /cm. Downstream site LD2 had higher conductivity values (up to 3'56µmho /cm), but this site is tidally influenced. Paradise Bay Basin Two unnamed tributaries (sites LD31 and LD41) of Paradise Bay were sampled in February (Figure 3). In April,. site LD41 was sampled a second time, but site LD31 could not be sampled due to lack of flow. On February 24, fecal coliform measured 13fc /10Oml at site LD31 (Table 5). Whereas, on the same date, site LD41 on the other tributary had a fecal coliform level of 124fc /100ml. On the next sampling date in April, site LD41 had a level of only 6fc /100ml. Based onta sample size of 2, site LD41 failed the second part of the fecal coliform standard; 50% of the samples exceeded 100fc /1OOml. Based on a single sample, loading at site LD31 was calculated to be 0.01 billion fc /day. Mean loading at site LD41 for the two dates was 0.92 billion fc /day. For both tributaries (sites LD31 and LD41), TSS ranged from 2.0 to 7.4mg /l, turbidity from 10 to 13 NTUs, temperature from 8.4 to 9.1 °C, and conductivity from 148 to 162gmho /cm (Table 5). 18 a a 3 O N d w N -4 N d .i LL ro N N 01 H a ro rn rn O+ c w x n� m s., U 3 0 a N G 0 ro U O N 01 x J-1 4J ro 'O m >a N ro tll N �1 0) O) ro ro 4 -4 ro CP M v id 11 O ro t7w 3:.,4 w co N. S2 ro H v p >. a > •.IUV M 4.) o p 0 o to a .O N O �ro N V C] a p a d >4 J-I ro M s4 p n. a F- U W — E+ � a a� in a 4 -M ' . M •O a p .., F a .a z >4 E� N p a .y. O a a M rol;N a 4-J N 'O O R.•M E N -4 _0 o N N N p a p a }.1 O M rl O O p ro w .•1 a U• ,4 -� v -4 U W O W U N a a v ro 'O r-I iL !r ro w t0 O M 01 tn d. h co rl M C v M tD tO 1n to to ON M r- V' r, t` tD to 111 O\ 1•1 111 -T in In N N M tD N Co to ON .--I ON tD co 14 M tD 1` tD 1` O to Ot ei r1 N N N P4 M M 14 co ON N rl aD M M tD In l� w N N r- 111 1` W tD .-1 '-1 00 0o I` M O\ M Ot r- h I. In c4 In to I n tD D1 O\ n 10 r4 M M r, In M c. t0 4 9 in 9 O I O M ,-4 01 O co • I O N Ot M tD In d' M 1 W N r1 O to tD N -T M In 1 r4 to tD C' O N 1 In N W tD co v n tD r, o% v rl 01 M M M M M 1` N N N N co tD N O N 4 N N N rl N N N W co tp tD O . M 1` r•1 M ri N ei In N N' I r o rl 1 In O cO M M tD N ri D1 M it „1 M M 1` M 1 In M N N to I tD to M co to O1 .•�- .� I d' 01 ri N '-I 01 .ti '•i N ON O1 e4 O1 N O1 O1 '4 •-i. 01 O1 01 0% 01 M r-4 01 1••1 01 01 rl '•I N m r-4 n m M '1 rl - (A O1 L 4 n I-q .•1 N a) N N N wQ ?d U O 4.) O) O Sa U 4 31 O+ Q > U G Cl Sa w m z o h w N d a N O z v a ro N N v ai 41 11 ai i � N ro >4 >s ro r- 04 •.1 s4 N .1r ro 11 N O .-I ro .-1 7 N N ,Q O > ro O (L) z w 4J C N O A. 0 .s~ C) N x a� ro ++ •-1 N N In I � O N v >v U 41 v -.4 '-I > '-I .,.1 0 .IJ U U O N s4 a tv O 3 U N ro LL � ro S.r N O yJ N ro p a� a 1: 41 H y .4 N Bywater Bay Basin Monitoring results of Bywater Bay's single, unnamed tributary stream, sampled at storm event site LD51, are shown in Table 5. Fecal coliform GMV for the two dates was 42fc /100ml. Both parts of the fecal coliform standard were met (Table 6). Mean fecal coliform loading was 0.49 billion fc /day. Mean TSS was 4.3mg /1, turbidity 6.3 NTUs, temperature 8.4 °C, and conductivity 150µmho /cm. Squamish Harbor Basin Ambient monitoring results of the sampling conducted at site LD1 on Shine Creek are shown in Table 9. The highest fecal coliform level occurred on January 27, 1992, the date associated with the greatest rainfall (Table 1). The dry season GMV was 11fc /100ml and the wet season GMV 17fc /100ml (Table 6). Both parts of the standard were met for both seasons. Mean loading was calculated at 0.91 billion fc /day for the dry season and 5.5 billion fc /day for the wet season. Over the entire study, TSS ranged from 0.6 to 5.9mg /l and turbidity from 0.5 to 7.9 NTUs. Conductivity ranged from 125 to 5900gmho /cm; the upper value indicates tidal influence at this estuarine site. Temperature ranged from 3.1 to 16.0 °C. This upper temperature, which occurred in August 1991, is the maximum allowed under the Class AA standard (Table 2A). Thus, the standard was barely met in August. The two unnamed tributaries (sites LD61 and LD71), sampled twice during storm events, had low levels of fecal coliform; GMVs for both streams were less than 9fc /100ml (Table 5). Mean loadings for each of these low -flow streams were less than 0.1 billion fc /day. Mean values for other parameters measured at site LD61 were: TSS 4.3 mg /1, turbidity 6.3 NTUs, temperature 8.40C, and conductivity 150 µmho /cm (Table 5). Mean values at site LD71 on the other tributary were: TSS 4.5mg /l, turbidity 11 NTUs, temperature 9.2 °C, and conductivity 175µmho /cm. 20 N 01 N 0) �-I T-1 O O � O r-4 a., .. z ON .,-1 0 U cn > U o .O r-1 •rf --� U1 O O r� O 4J O N Co O N r- M 111 O U1 r-1 rr U L o1 Co U1 N N %0 ON U7 10 04 J rq H H r1 H r1 U1 LO I~ f0 O 'O U rn X S-1 4l O 4-3 O O U -� 4J r-1 p r-I l- t- r-1 U M rZ v O 3 O O H •. N O >1 H 4J A aH In ; co H d. qr 0) LO co D N -r+ Ei ri 4 4 r.; r� 9 o ri a) J0 z o W ," z W E-4 lz 0 4-) v rl 'O rl O N r-I W a% co N fA t0 En VI 0) 14 f0 M }J (D rO r-4 l 1; N 14 ri O In N 4 O -04-H r-i E ON 0 O U1 •r-4 U1 N f0 �w m +J 9: 7y O -r-1 .-. aJ 0 r-4 •H w 3 w o -r4 c0 O r4 O o 4.7 044 c0 44 r-1 %o I d• LO N co %D w lw U Ul U- 4\ r-1 I N �4 O1 O ?� N r-i U b •r4 -H w p O U to 1= :3 O r-I 'O 4J ON 01 r-1 01 N. ON Rf �1 f0 ON ri ri Ol cn OV O1 GI U r-1 r-i o1 O1 r-I O\ rl 41 41 O O rn O1 r-1 r-4 N ".� M C1 r-1 r1 r, 01 O7 4J N b r! LA ri r- r-1 r-1 N ON ri Q r♦ O >1 is p iL N G1 N r-I O .0 N � c0 r-1 04 O -W N I.1 U Ea O F J� f4 IS i]. > U II >a tO t~ as N (d 41 O N c0 O ca E-4 4J t!1 w z Q cn z ?1 DISCUSSION Oak Bay Basin Mats Mats Bay All three of Mats Mats Bay's tributaries sampled under our ambient monitoring program violated the Class AA standards for fecal coliform. Concentrations were higher in the dry season (GMV 115 -123 fc /100ml) than in the wet season (GMV 10- 35fc /100ml). In two of the tributaries (LD6 and LD7), fecal coliform mean daily loadings were as high or higher in the dry season as they were in the wet season despite the 4 -5 times greater wet season flows. In Rubida's 1988 study (1989), fecal coliform concentrations were only slightly higher in the dry season (GMV 52 -55 fc /100ml) than in the wet season (GMV 23 -39 fc /100ml) for the three tributaries, based on 2 dry season samples and 2 -3 wet season samples. Based on a sample size of two, the dry season GMVs slightly exceeded the Class AA standards. In a 1989 study, Smayda and Harper (1989) reported that the flow- weighted GMV for all three tributaries combined was 230 fc /100ml in the dry season; no wet season data was collected. This level exceeds the Class AA standard. Several monitoring studies have been conducted on the marine water of Mats Mats Bay. In 1987, based on 13 samples collected from May to July, Harper -Owes (1987) reported a fecal coliform GMV of 4 fc /100ml, well below the 14 fc /100m1 limit for Class AA marine water. In another 1987 study, conducted during 1 week in September, the Washington Department of Social and Health Services (now Department of Health or DOH) sampled 13 stations in Mats Mats Bay four times. Three of these stations failed the second part of the Class AA standard (unpublished data). From March 1988_ to January 1989, Rubida (1989) collected 50- samples at 4 stations in the bay. Fecal coliform GMVs were 2 fc /100m1 or less at the 4 stations. Both parts of the standard were met. In 1989, Smayda and Harper (1989) collected 28 samples at 4 marine stations from July to October. The GMV for all samples was less than 2.5 fc /100ml. Both parts of the standard were met. Since 1989, DOH has been collecting samples at 15 stations in Mats Mats Bay under an ambient monitoring program. Based on 71 samples collected in 1989, fecal coliform levels met Class AA standards at all 15 stations (unpublished data). No samples were collected in 1990. In 1991 -92, when 195 samples,were collected, fecal coliform levels violated the standard at 6 of the 15 stations (DOH, unpublished data). During the September 1991 sampling period, when the weather was "dry" and "about 20 boats were moored in the bay, 42 of the 90 samples collected exceeded the 14 fc /100ml standard (Gerald Lukes, DOH, personal communication). 22 Elevated fecal coliform levels may be partly due to the morphometry of Mats Mats Bay and its circulation pattern. Mats Mats Bay is almost totally closed. Water enters and.exits the bay through a long, narrow channel, approximately 3300 ft. by 300 ft. Smayda and Harper (1989) calculated that bay water is exchanged at the rate of 1.3 times each day. However, despite this great exchange rate, they observed that surface current was toward the bay regardless of tidal direction, when even light (2- 5 knots), northerly breezes occurred. They surmised that the light winds which prevail during summer and fall serve to hold floating and near - surface objects within the bay. From temperature and salinity measurments, Smayda and Harper ascertained that vertical mixing did not occur from July through September, but did occur in October. Thus fecal coliform occurring in freshwater, which floats on top of the denser saltwater, could become concentrated in Mats Mats Bay surface water, at least during the summer months. Limited shellfish data exists for Mats Mats Bay. Six oysters collected from July to October 1989 had a fecal coliform GMV of 333 fc /100ml (Smayda and Harper 1989). This exceeds the 230 fc /100gm guideline promulgated by the U.S. Food and Drug Administration regulating commercial shellfish sales. Oysters collected near the stream mouths displayed greater concentrations (GMV 552 fc /100gm) than did samples collected from a more distant point (GMV 259 fc /100ml). Other data include one shellfish sample collected in June 1985 having 20 fc /100gm and three in 1987 having concentrations of 20, 78, and 1300 fc /100gm (unpublished data, Jefferson County Department of Health). The concentration of fecal coliform in the freshwater, surface layer, could help explain the elevated fecal coliform levels in the oyster samples. Oysters, which filter large volumes of water each day, concentate algae and microorganisms including bacteria. Because they inhabit the intertidal zone, oysters are highly exposed to surface water. What are some of the possible causes of the elevated fecal coliform levels in the Mats Mats Bay tributaries? The LD6 drainage is primarily uninhabited forestland. However, there are a few homes in the area which are possible sources. Forestland predominates in the LD7 drainage. A few houses on Verner Road could potentially convey pollutants via the ditches along Verner Road and West Mats Mats Road. The potential for pollution is increasing on the hillside above Mats Mats Road; building sites are now being cleared. Several streamlets, draining this hillside, empty into the ditch on West Mats Mats Road The LD8 drainage originates on the west side of Oak Bay Road. Two wetlands, one in an alder forest, the other in an adjacent clearcut, are headwaters for the drainage. Flows from these wetlands pass under Oak Bay Road through separate culverts. These two headwater sources, and a third water source originating on the north side of Mats Mats Beach Road, all flow through a horse farm. Although to a large extent dense underbrush forms a natural barrier to the streamlets, some direct animal access or contaminated surface runoff probably contributes to the elevated 23 fecal coliform levels at site LD8. From site LD8 at Bay Shore Drive, the stream flows for about 1,000 feet through a pasture before emptying into Mats Mats Bay.. The stream is unfenced in this pasture. Two dirt - covered culverts provide the animals passage across the stream. Two or three horses usually occupy the pasture. The only home which could possibly be a source of pollution to this tributary is located on Mats Mats Beach Road. The fourth Mats Mats Bay tributary (LD21), sampled twice during storm events, showed almost no fecal coliform bacteria present. This small stream flows through forestland, where there are presently no homes or domestic animals. No previous water quality data exists for this tributary. Oak Bay Fecal coliform levels in the unnamed tributary (LD11) to Oak Bay met Class AA standards. There are no domestic animals upstream of the sampling site and only two homes near the stream. No other data is known to exist for this small (2 cfs) stream. DOH sampled five stations in Oak Bay from 1988 to 1992 under their ambient monitoring program. Fecal coliform GMVs were less than 2 fc /100ml at all five stations (16 -28 samples per station) during this period (DOH, unpublished data). Thus, marine Class AA standards were met. Ludlow Bay Basin In Ludlow Creek, fecal coliform levels were highest during the dry season when Class AA violations occurred at downstream site LD2 (GMV 66 fc /100ml) and upstream site LD4 (GMV 601 fc /100ml). Wet season GMVs were less than 12 fc /100ml at all three Ludlow Creek sites. However, wet season data from site LD4 failed the second part of the standard. Rubida (1989) sampled the same three sites in 1988 -89 and found that site LD4 had a dry season GMV of 68 fc /100ml, which was about 3 times greater than the wet season GMV and a violation of the Class AA standard. No substantial differences were observed for wet and dry seasons at the other two sites; GMVs were less than 36 fc /100ml. - Smayda and Jones (1991) summarized Ludlow Creek water quality data collected in 1984 and 1989. Fecal coliform levels measured on three dates (March, May, and September) in 1984 ranged from 23 to 33 fc /100ml. In 1989 fecal coliform samples collected once each month from June to October had levels mostly between 14 and 33 fc /100ml except for the August sample, which had a level of 80 fc /100m1. The highest fecal coliform levels occurred at upstream site LD4. The stream forks approximately 50 feet upstream of this site at Embody Road (County section). The west fork which drains a forested hillside, contributes most of the flow to the main stem. Between the forestland and the main stem, the west fork flows through about 1,000 feet of unfenced grazing land through two separate pastures. The pasture upstream of Embody Road 24 (private section) did not appear to be recently used. The pasture downstream of Embody Road (private section) contained cow manure, but no animals were observed. The stream channel in this pasture is not well defined. It is likely that this stream overflows its channel during the rainy season. The north fork upstream of Embody Road (County section) drains agricultural lands in Beaver Valley for a distance of about 1 mile. The stream through this section has been channelized and has a very low gradient and slow flow. The stream appears fenced for the most part and is hidden from view by a dense canopy of herbs and shrubs. Between site LD4 on Embody Road and site LD3 on Beaver Valley Road, the stream flows through forested wetlands with beaver dams and standing water. It is probably due to this large wetland habitat that fecal coliform levels decrease from site LD4 to site LD3. This same kind of wetland habitat extends another 0.4 miles downstream of site LD3 on Beaver Valley Road before the gradient increases and the stream takes on a more characteristic stream - like appearance. From site LD3 to site LD2 on Paradise Bay Road, the stream flows through forestland, where there are no homes or domestic animals. This would explain the similar fecal coliform levels at these two sites. Site LD2 is located on the estuary. Seepage of saltwater from soils previously inundated with tidal water probably accounts for the slightly higher conductivity measurements at this site compared to the two upstream sites. Fecal coliform levels in Port Ludlow Bay have generally met the 14 fc /100ml State standard. In 1984, GMVs ranged from <3 to 8 fc /100ml at it sites in the Bay (Patmont et al. 1985). The highest GMVs occurred near the Wastewater Treatment Plant discharge (4 fc /100ml) and at the marina (8 fc /100ml). In monthly sampling conducted by Rubida (1989) from March 1988 to January 1989 at four sites in Port Ludlow's Inner Bay, GMVs for the 4 sites ranged from 1.1 to 4.0 fc /100ml. In a different study, conducted in 1989 from June to October, 38 of -the 50 samples collected at five sites contained less than the detectable limit (2 fc /100ml), and the remainder had less than 6 fc /100m1 (HLA 1990). The upgrading of the Wastewater Treatment Plant in 1989 resulted in a substantial decrease in the fecal coliform concentration of the effluent. In 1984 the fecal coliform GMV was 77 fc /100ml (Patmont et al. 1985) ; in 1989 the GMV was. 11 fc /100m1, one - seventh the 1984 concentration (HLA 1990). Several studies have indicated that boaters are a cause of elevated fecal coliform levels in Port Ludlow Bay. Patmont et al. (1985) reported that of the 11 sites sampled throughout Port Ludlow Bay from March to November 1984, the highest fecal coliform GMV (8 fc /100m1) occurred in samples from the site closest to the marina. Furthermore, the GMV for the marina site was about 13 times higher from May 28 to September 4 (51 fc /100ml), when 105 to 107 boats were moored in the Bay, than it was from March 16 to April 10 (4 fc /100ml) , when less than three boats were in moorage. The authors reported that 15 percent of 25 the shellfish samples collected in 1984 during summer weekends had concentrations exceeding 230 fc /100gm. In a separate study conducted in 1985, Patmont et al. (1985) compared fecal coliform levels in water and butter clams to the number of boats moored in the Bay during the Fourth of July holiday period. They found that fecal coliform levels in both the water and clams increased and decreased in a manner corresponding to an increase and decrease in the number of moored boats. Fecal coliform GMVs exceeded the State standards for marine water on four of the eight days sampled. About 20 percent of the clams sampled had fecal coliform concentrations above 230fc /100gm. Rubida (1989) reported that high fecal coliform concentrations in the Inner Bay were associated with increased boating activity during the 1988 Fourth of July weekend. The GMV for the eight sites sampled was 9 fc /100m1; the three highest measurements were 37, 75, and 171 fc /100m1. Faust (1982) reported that fecal coliform in shallow bays increased from 3 to 28 fc /100ml after the arrival of boaters on the Labor Day weekend, and decreased soon after they departed. Paradise Bay Basin The unnamed tributary sampled at site LD31 is better described as a ditch which runs along the last 0.2 miles of Tala Shore Drive. Several streamlets draining the hillside flow into it. There was very little flow in this ditch in February and none in April. No houses or domestic animals were observed. The other unnamed tributary sampled at site LD41 is a typical small stream and flows all year. Based on only two samples, this stream failed to meet the second part of the fecal coliform State standard. The stream originates in forestland (recent clearcut) and then flows through a populated area, the Paradise Bay Community. Septic effluent is the most likely source of the elevated fecal coliform level (124 fc /100ml) measured in February -1992. DOH does not monitor Paradise Bay under their ambient monitoring program. No marine data for this embayment was found. Bywater Bay Basin The single tributary of Bywater Bay Basin, sampled twice during storm events, passed the Class AA standard, but not by a wide margin; the fecal coliform GMV at site LD51 was 42 fc /100ml. This small stream drains forestland in which there are no houses or domestic animals. DOH sampled three stations in Bywater Bay a total of 22 times each from 1988 to 1992. Fecal coliform GMVs for this time period were less than 2.0 fc /100m1 at all threa.stations (unpublished data). Thus, the marine Class AA standard was met. 26 Squamish Harbor Basin Fecal coliform levels in Shine Creek, the largest of the basin's drainages, were low in both dry (GMV it fc /100ml) and wet (GMV 17 fc /100ml) seasons. Fecal coliform levels would be expected to be low in this stream which drains forestland having no homes or domestic animals. The stream temperature (16.0 °C) at site LD1 in August 1991 barely met the Class AA standard, which states that temperature shall not exceed 16.0 °C due to human activities. Three factors may explain the high temperature. First, Shine Creek passes through a series of wetlands in which beavers have created several wide, shallow impoundments. These impoundments have no substantial tree canopy and are open to sunlight penetration. The dark, organic bottom and tanin- stained water contribute to the heat absorption. Second, Shine Creek and a small tributary, Redtail Creek, flow through a clearcut area upstream of Route 104. Although stream buffers have been left, some sunlight penetration may be occurring. Third, stream warming apparently occurs in the golf course ponds at the headwaters of Shine Creek (see following discussion). One could conceivably attribute the high temperature at this estuarine site to tidal water coming in over a sun - warmed mud flat. However, the fact that, at the time of sampling, conductivity was 152 µmho /cm or only 0.4 percent of the conductivity of sea water, this explanation would have to be disallowed. The temperature standard refers to increases "due to human activities." Thus, forest management and golf course management are the two most likely human activities affecting stream temperature_ A temperature- related observation was made on Shine Creek on July 2, 1991, when County water quality staff were characterizing riparian habitat. While working a stream section upstream of Route 104 (not a regular monitoring site), the survey crew noted a sudden 4 to 6 inch rise in the water level and a change in water transparency from clear to turbid. Within l minute, they observed the water temperature change from 13.3 °C to 15.5 °C. This increase of 2.2 °C exceeded the maximum allowable increase of 1.3 °C for a background temperature of 13.3 °C (Table 2A). The increase was attributed to the rapid release of water from the golf course pond. The release also caused some bank erosion and bottom siltation. Oxygen levels have not been reported in this study due to a faulty instrument. No definite conclusions can be made. However, oxygen levels at site LD1 appeared low on some dates compared to measurements taken at other sites.= This could be due to the lack of aeration in the slow moving, wetland section immediately upstream of site LD1, and to the decomposition of organic material in the wetland. Worst case conditions would occur at sunrise before photosynthesis counteracts nighttime oxygen consumption by the aquatic community. From a fisheries standpoint, Shine Creek probably offers the greatest potential as a salmon producer of all the streams in the Ludlow Watershed. Thus, best management practices of this drainage should be encouraged so that streambed siltation and excessive stream temperatures are minimized. Future monitoring - could include the use of continuous- recording thermographs to better evaluate temperature conditions. Dissolved oxygen levels should be further investigated. Some of the conductivity measurements (617 -5900 µmho /cm) taken at estuarine site LD1 have obviously been affected by the tidal water. Even some of the lower readings (Table 9) are probably slightly higher than normal due to seepage from saltwater - saturated soils. DOH sampled seven stations in Squamish Harbor a total of 21 times each from 1988 to 1992 under their ambient monitoring program. Fecal coliform GMVs at the seven stations ranged from 1.9 to 2.5 fc /100ml (unpublished data); all were well within the Class AA standard for marine water (14 fc /100ml). 28 REFERENCES Anon. 1989. Guidance for conducting water quality assessments. Washington Department of Ecology, Publ. No. 89 -28, Olympia, Washington. APHA. 1980. Standard methods for the examination of water and wastewater, 15th ed. American Public Health Association, Washington, D.C. - DOH.1990. Shellfish tasks for the Puget Sound Ambient Monitoring Program. Department of Health 1990 technical report, Olympia, Washington. Faust, M. 1982. Contribution of pleasure boats to fecal coliform concentration in The River Estuary, Maryland, USA. The Science of the Total Environment 25:255 -262. HLA. 1990. 1989 water quality conditions of Port Ludlow Bay. Harding Lawson Associates /Harper -Owes, Seattle, Washington. Harper -Owes. 1987._ Proposed Seattle Yacht Club outstation facility, Port Ludlow, water quality assessment. Harper -Owes, Seattle, Washington. Patmont, C.R., G.J. Pelletier, and M.E. Harper. 1985. Water quality investigation of Port Ludlow. Harper -Owes, Seattle, Washington. Rubida, P. 1989. Jefferson County ambient water quality report. Jefferson County Planning and Building Department, Port Townsend, Washington. Smayda, T.J. and M.E. Harper. 1989. circulation and water quality of Mats Mats--Bay. HLA /Harper -Owes, Seattle, Washington. Smayda, T.J.'and C. Jones. 1991. Water quality investigation in Port Ludlow Bay, 1990 nonpoint source study. Harding Lawson Associates, Seattle, Washington. Zar, J.H. 1984. Biostatistical analysis, 2nd ed. Prentice - Hall, Englewood Cliffs, New Jersey. 29 APPENDIX A QUALITY CONTROL QUALITY CONTROL Field Replicates Field replicates of those parameters measured with the Water Analyzer (temperature, conductivity, pH, and dissolved oxygen) as well as of flows were taken at the sampling sites. Two sets of measurements were taken within a few minutes of one another. Replicate water samples, collected in separate bottles within a few minutes of one another, were taken for fecal coliform, total suspended solids, and turbidity. Replicate measurements provide an estimate of the random variability (precision) in the results due to the instrument and its use. The analysis of replicate samples provides an estimate of the variability due to sampling and analysis. The results for different parameters will exhibit different levels of variability due to the nature of the measurement, sampling and /or analytical process. The variability in fecal coliform results exhibits a log normal distribution. The standard deviation is an estimate of the absolute variability of the results and usually increases with the magnitude of the results. Precision is usually reported as relative standard deviation (RSD). The RSD (or coefficient of variation) is usually inversely proportional to the magnitude of the rusults. The RSD is given by: s RSD ( %) = x 100 X where s is the estimate of the standard deviation of the individual results and X is the mean of the replicate results (Zar 1984). For duplicate results, this can be written as: H1 2 RSD% = x 100 X where IDI is the absolute difference between the.two values. Field replicate results of the parameters measured in this study are shown in Table A -1. Dissolved oxygen and pH results were determined to be unreliable due to a defective probe and are not reported. 31 Table A -1. Quality control field replicate results of parameters reported in this study; "Dif." is the absolute difference between values and "RSD" is the relative standard deviation in percent. Date Site Rep.1 Rep.2 Dif. RSD Rep.1 Rep.2 Dif. RSD 0.5 1.6 Fecal coliform(fG100mL)I. 74.1 Row(cfs) 8 -22 -91 LD4 130 190 60 5.3 - - - - 9 -17 -91 LD2 57 160 103 16.0 - - - - 11 -25 -91 LD6 13 8 5 14.8 - - - 12 -17 -91 LD4 6 2 4 62.5 - - - - 1 -27 -92 LD1 94 98 4 0.6 - - - - 2 -19 -92 L07 10 9 1 3.3 - - - - 2 -24 -92 LD6 140 130 10 1.1 0.16 0.15 0.01 4.56 3 -09 -92 LD4 1 3 2 141.4 - - - - 4 -22 -92 LD41 3 11 8 52.6 0.18 0.19 0.01 3.82 Temperature( C) Conductivity(umholcm @ 25 C) 3 -13 -91 Total Suspended Solids(mg/L) 5.8 Turbidity(NTU) 9 -17 -91 LD2 0.5 1.6 1.1 74.1 2.5 2.5 0.0 0.0 11 -25 -91 LD6 5.8 6.0 0.2 2.4 9.7 10.5 0.8 5.6 12 -17 -91 LD4 3.8 3.6 0.2 3.8 4.7 5.0 0.3 4.4 1 -27 -92 LD1 6.2 5.6 0.6 7.2 8.0 7.8 0.2 1.8 2 -19 -92 LD7 7.0 6.4 0.6 6.3 22.0 22.0 0.0 0.0 2 -24 -92 LD6 14.2 13.2 1.0 5.2 20.0 20.0 0.0 OA 3 -09 -92 LD4 3.2 4.2 1.0 19.1 4.5 5.0 0.5 7.4 4 -22 -92 LD41 7.6 7.2 0.4 3.8 11.0 11.0 0.0 0.0 Temperature( C) Conductivity(umholcm @ 25 C) 3 -13 -91 LD3 5.8 5.7 0.1 1.2 111 115 4 2.5 8 -22 -91 L01 16.0 15.9 0.1 0.4 154 150 4 1.9 8 -22 -91 LD4 12.2 12.1 0.1 0.6 142 143 1 0.5 9 -17 -91 LD6 13.7 13.6 0.1 0.5 190 192 2 0.7 11 -21 -91 LD 6 8.1 8.2 0.1 0.9 198 191 7 2.5 12 -17 -91 LD4 5.3 5.3 0.0 0.0 161 166 5 2.2 2 -19 -92 LD3 5.7 5.7 0.0 0.0 125 126 1 0.6 2 -19 -92 LD6 6.4 6.4 0.0 0.0 160 161 1 0.4 2 -24 -92 LD6 8.4 8.4 0.0 0.0 134 135 1 0.5 3 -09 -92 LD4 6.4 6.6 0.2 2.2 150 152 2 0.9 4 -22 -92 LD6 9.6 9.6 0.0 0.0 186 186 0 0.0 4 -22 -92 LD41 9.1 9.0 0.1 0.8 162 161 1 0.4 RSDs for fecal coliform are based on natural logs - of the results. 32 Duplicate temperature measurements agreed within ± 0.2 °C, and usually within ± 0.1 °C. RSDs were less than 3 %. Duplicate conductivity measurements agreed within ± 7µmho /cm; RSDs were less than 3 %. Duplicate TSS values agreed within ± 1.1 mg /l; RSDs were less than 8% except in two instances (19.1% and 74.1 %). Duplicate turbidity values were within ± 0.8 NTUs; RSDs were less than 8 %. Flow measurements were replicated on only two dates. The volumetric method was used at site LD6 on February 24, 1992, and the current meter method was used at site LD41 on April 22, 1992. In each case the difference between replicates was 0.01 cfs and the RSDs were less than 5 %. Seven of the nine fecal coliform field replicates had differences of 10 fc /100ml or less; the other two differences were 60 and 103 fc /100ml. The RSDs of the natural logs ranged from 1 to 141 %. Quality Control Checks QC checks are "known" samples, supplied by the Quality Assurance Section of DOE, to be analyzed with the usual run of samples. Thus, by comparing the analytical results obtained for the QC checks to their true values a measure of accuracy is obtained. All QC checks analyzed in this study for TSS and conductivity were found to be within acceptable limits (Table A -2) . Discussion- Except for fecal coliform, variability for all other parameters as determined from the replicate analyses was low and acceptable. Accuracy for TSS and conductivity was also acceptable. QC checks for the other reported parameters are not available. Fecal coliform duplicates indicated occasional moderate variability (Table A -1) . Fortunately, the variation was relatively low (< 10 fc /100ml) for values less than 50 fc /100m1, the Class AA standard. This variation is typical for fecal coliform. It points out the need to exercise caution in interpreting -data close to a State standard, especially when the GMV is based on a small sample size. It also points out the advantage of having a large sample size for determining trends and differences between sample sites. 33 O C r b+ >r r- r-q r-I-I r-4 ,—Oi 4-) - -ri R ( O 0 �S7r >1 :3 r-4 m m r4 :1 )a (W (1 S Q) Q) Q) Q) or +' Q) r a>i U U o U U (13 a 04 04 •� .r4 4 �4-) � rd+°� ftJ •rl rL3 In N O N O 4J ri Q) IT 4-) .4 ar W C •rl N rn N to d' O c�1 tyr C1 crr O 04 r. .,4 co >r to i~ w r I ch of m a% 1 U O co - U 3 tc1 d; tr1 co II U U id U Q) (tf U Q) >r CJ 4J Ot 0 Q) b r-1 r-f N •rj 44 W O 44-) r-1 c" 1 tG cn W U O 44 PQ -H ri 0 crf >c M dr M 41 N CN O to 44 Q) r-1 rcy 1 " co -44j O U N ch > I RS 0 4J 14 v c4 dr c4 � � N C7 N C7 � ko r--1 O Q) a i✓r a i to v e o41 a�i a� r- 0 r o, . -- 0-1 r. w (13 E4 N v N IV E1 > O U� WO2T )-1 T3 G Q) C • 4 1340 4 U r-1 (0 4J 0) N O ra 3 -*i 41 O r. co w r. I~ P r-i N V N M r!') 4J W Q) -H O c0 t� !~ .0 r-4 m > to 41 U ro Q) pC, -ri W r-'1 >4 r-4 O Q +J O to -.-1 N C 4j O r—r O ad f.. rtt to -rl Q) Q) 4J U Q) I; Q) M U 04 ri N ri N N Q) m Or 0 • 04 rd ((f cl to O U c�0 1� to s: N N N N N to Ol Cl a% C% m r-4 Q) C% r4 Rf :3 0 QI L` N N N 44-1 N r-I 41 N N N N N Rt O to f0 c13 I I 1 I I E4 4J P4 > O ri ri v lw d' 34 1 APPENDIX B SAMPLE SITE LOCATIONS SAMPLE SITE LOCATIONS LD1 Shine Creek - Upstream side of three concrete culverts on South Point Road. LD2 Ludlow Creek - Upstream side of concrete culvert on Paradise Bay Road. LD3 Ludlow Creek - Upstream side of culvert on Beaver Valley Road, about 300 feet south of intersection with Oak Bay Road. LD4 Ludlow Creek - Upstream side of culvert on Embody Rd. (County section) off Beaver Valley Road. LD6 Unnamed - Downstream side of culvert on Verner Road off Oak Bay Road. LD7 Unnamed - Downstream side of culvert on West Mats Mats Road off Verner Road. LD8 Unnamed - Downstream side of culvert on Bay Shore Drive off Oak Bay Road; near driveway to horse farm. LD11 Unnamed - Upstream side of culvert on Hiller Drive off Oak Bay Road. LD21 Unnamed - About 25 feet downstream of culvert on Oak Bay Road; next to old growth stump; about 0.25 miles south of intersection with Bay Shore Drive. LD31 Unnamed (ditch) - About 12 feet upstream of culvert at end of Tala Shore Drive (County section); near fire no. 830. LD41 Unnamed - About-15 feet from downstream side of culvert at 61 Shore Drive (junction of East Maple Road);. samples taken at upstream end of a concrete stream channel. LD51 Unnamed - Upstream side of culvert on Seven Sisters Road off Paradise Bay Road. LD61 Unnamed- Stream is about 100 feet east of boat launch at Hicks County Park off Shine Road; samples taken about 25 feet downstream of riprap on beach. LD71 Unnamed - Downstream side of culvert at 1023 Shine Road; flow taken on beach about 50 feet from culvert. 76